|Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that enables ultra-sensitive assays for chemicals and biochemical. Enormous progress of SERS has been made in various fields based on the rapidly developing syntheses and applications of nanomaterials. SERS has been successfully used for a number of sensing applications, including the sensing of heavy metals, disease biomarkers and biological molecules. In particular, SERS-based biosensors have received extensive attention in the field of disease diagnosis.
We have recently reported SERS-active substrates for detection of biomolecues and their versatile applications in highly sensitive SERS-based bioassay. SERS-based MTT assay for living cell viability in situ and the effect of the enzymatic reaction on the spectra of a GOx-conjugated SERS-active reporter molecule (4-mercaptobenzoic acid (MBA)) for glucose sending were explored. A facile SERS sensor based on a silver-ion-mediated amplification effect, which was designed for the determination of glucose concentration, was also reported. Fabrication of novel compound SERS substrates composed of silver nanoparticles and porous gold nanoclusters was also reported. This new type of SERS substrate was used for ultrasensitive detection of a physiological metabolite of urea molecules.
In this presentation, the potential, utility and versatility of ultrasensitve SERS-based bioassay will be demonstrated.