|Microfluidics chip platform can provide convenient and accurate diagnosis and analysis tools. In this talk, we present recent developments in microfluidics, with special emphasis on disposable plastic devices format, embedded valve, and various nano/microstructures for biosensor. In detail, we overview of the common methods used in the fabrication of polymer microfluidic systems, including replica and injection mold¬ing. Especially, we will explain the different methods by which on-chip operations—such as the pumping and valving of fluid flow, the mixing of different reagents, and the separation and detection of different biochemical species—implemented in a microfluidic format. Furthermore, we propose a novel and promising platform of “Bio-Sensitive Nanotopological Trap (b-SNAP)” for high efficient capture, isolation, and identification of life-threatening diseases. We believe that b-SNAP, consisting of 3D hierarchical organic-inorganic hybrid nanostructures, provides selective pathogenic recognition and capture by controlled surface chemistry and efficient 3D nano-bio interface adhesion, leading to much more increased capture performance than conventional antibody-based immunoassay systems. In addition, we will develop a “Self-assemblable Bio-device (SAAB)” incorporated by b-SNAP, extracting nucleus acids, polymerase chain reaction (PCR), and digital genetic analysis, which can offer complete identification of diseases in a single cell level.